Endolysin PlyC Binding to Model Membranes Reveals its Entry Point on Mammalian Cells
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منابع مشابه
Abstract Title of dissertation: ENGINEERING ENHANCED STRUCTURAL STABILITY TO THE STREPTOCOCCAL BACTERIOPHAGE ENDOLYSIN PLYC
Title of dissertation: ENGINEERING ENHANCED STRUCTURAL STABILITY TO THE STREPTOCOCCAL BACTERIOPHAGE ENDOLYSIN PLYC Ryan Daniel Heselpoth, Doctor of Philosophy, 2014 Dissertation Directed by: Associate Professor Daniel C. Nelson Institute for Bioscience and Biotechnology Research and Department of Veterinary Medicine Antibiotic misuse and overuse has prompted bacteria to rapidly develop resistan...
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Makkonen and his colleagues published an article in the Journal of Virology entitled “6-Oand N-Sulfated Syndecan-1 Promotes Baculovirus Binding and Entry into Mammalian Cells” (1). Some parts of the data and conclusions seem misleading and not fully justified. Heparan sulfate (HS) alone can bind baculovirus to cells during entry and transduction. Syndecan-1 (SDC-1) contains a protein core and H...
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PlyC, a bacteriophage-encoded endolysin, lyses Streptococcus pyogenes (Spy) on contact. Here, we demonstrate that PlyC is a potent agent for controlling intracellular Spy that often underlies refractory infections. We show that the PlyC holoenzyme, mediated by its PlyCB subunit, crosses epithelial cell membranes and clears intracellular Spy in a dose-dependent manner. Quantitative studies using...
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OBJECTIVES Streptococcus pyogenes, or Group A streptococcus (GAS), has a propensity to colonize human tissues and form biofilms. Significantly, these biofilms are a contributing mechanism of antibiotic treatment failure in streptococcal disease. In this study, we evaluate a streptococcal-specific bacteriophage-encoded endolysin (PlyC), which is known to lyse planktonic streptococci, on both sta...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2016
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2015.11.3083